The scene of Herod's banquet concludes the story of St. John the Baptist. It is set within a majestic, classical-style hall with a painted arch. The barrel vault resembles that of the Basilica of Maxentius in Rome. Two tables along the sides underline the perspective-based composition: the women sit at the left one, while the men are seated at the right. Behind the women is a group of musicians.
At the table in the centre is Herod, with an open window behind him. In the foreground Salome is portrayed performing her dance. Other men (including a dwarf) are looking on the left, where a servant is handing over John's head to Herod. A man nearby is making a gesture of disgust at the sight. The scene is inspired by the work of Filippo Lippi in Prato Cathedral, but is of lesser dramatic quality; the artwork was most likely provided by Ghirlandaio's workshop almost entirely.