The Marriage of the Virgin is set in beautiful Renaissance architecture, while the composition of the scene is rather traditional. In the centre is the temple priest, with the same features as in the Presentation. He is sealing the marriage between Joseph and Mary. To the sides are two processions, with men on the left and women on the right. Some of the former, angry at having not been chosen to marry Mary, are shown while breaking their sticks or raising their fists (a story originating in apocryphal legends of the life of Mary). Joseph's club, which had been chosen as the most vigorous, is barely visible over his shoulder. Most of the portraits are summary in style, apart some very carefully executed ones near the priest.
It is not clear if the shorter figures in both lower corner are children or have a different symbolic meaning. A preparatory sketch for this scene has been preserved in the Gabinetto dei Disegni e Stampe in the Uffizi, in which the priest in the centre is absent.